STUDIES ON THE GENE EXPRESSION IN DIFFERENT ALUMINUM-RESISTANCE PINEAPPLES

Autores/as

  • Yong-Hong Lin

Resumen

ABSTRACT
Pineapple (Ananas comosus (L) Merrill) is one of an economically cultured crops in Taiwan. It is normally cultivated in soils
containing strong acidity where aluminum (Al) often inhibits the growth of crop roots. This research was carried out with the
objective of identifying the main genes that directly influence aluminum tolerance by resistant and non-resistant pineapples
(Cayenne and Tainung No. 17, respectively). For this reason, both cultivars were grown in hydroponic nutrient solution, each
containing 0 and 300 μM AlCl3, in a growth chamber for four weeks. Samples of root apices were taken, followed by extraction of
RNA, selection of primers, sequencing and processing of PCR. Finally, after examination of genes, the relative expressions of
them for both cultivars were determined by quantitative PCR. The results obtained from polymerase chain reaction experiments
proved the Al-resistance characteristics of pineapples are mainly regulated by two genes, namely GAPDH (glyceraldehyde-3-
phosphate-dehydrogenase) and FBA (fructose biphosphate adenosine). This result may provide important indications for the study
of advancing genetic mechanisms on Al-resistance in pineapples.

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Publicado

2020-09-01

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